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HPLC Blood Test: Costs, Purpose, Normal Range and Interpretation

HPLC Blood Test
  • Sample Type : Blood
  • Parameters : 1
  • Report Time : 24/48 Hrs
  • Home Collection: Yes
  • Fasting : Not Required
  • Age Group: All
OFFER PRICE:  ₹798 ₹1000 30% off


HPLC Blood Test
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The HPLC Blood Test, or Hemoglobin Variant Analysis Test, is a laboratory test used to identify and measure abnormal hemoglobin variants in the blood. Hemoglobin is a protein in red blood cells that carries oxygen throughout the body. Normal hemoglobin comprises four protein subunits, each containing a heme molecule that binds to oxygen.

Abnormal hemoglobin variants can arise due to genetic mutations that alter the structure or function of hemoglobin. Some variants can cause diseases, such as sickle cell anemia, thalassemia, and other hemoglobinopathies. The HPLC Blood Test uses high-performance liquid chromatography (HPLC) to separate and identify different types of hemoglobin based on their size and electrical charge.

Learn everything about hb HPLC Test before you book lab test online. Understand the why, how, and what of this test.

Why Do I Need HPLC Blood Test?

If you are wondering why you need the HPLC blood test, also known as Hemoglobin Variant Analysis Test, it may be ordered by your healthcare provider for several reasons, including:

  • To screen for genetic hemoglobin variants in people of specific ethnic backgrounds or those with a family history of hemoglobin disorders.
  • To diagnose specific types of hemoglobinopathies, such as sickle cell anemia or thalassemia.
  • To differentiate between different types of anemia that may have similar symptoms.
  • To monitor treatment effectiveness in people with hemoglobinopathies or other blood disorders.
  • Assess the risk of complications during surgery or other medical procedures that may affect oxygen delivery to the body.
  • To confirm a suspected diagnosis of hemoglobinopathy.

How To Prepare For Hemoglobin HPLC Test?

To prepare for the HPLC blood test, also known as Hemoglobin Variant Analysis Test, you should follow these steps:

  • Consult with your healthcare provider about any medications or supplements you currently take. Some medications or supplements may interfere with the test results and must be stopped or adjusted before the test.
  • You need to fast for a certain period before the test. It may differ depending on the type of test you have been asked to get. Generally, you may have to fast for  8-12 hours before the test.
  • Drink plenty of water before the test to stay hydrated, as this can make it easier to draw blood.
  • Wear a short-sleeved shirt or clothing that allows easy access to your arm, as the doctor will draw the blood from a vein.
  • Avoid strenuous exercise or physical activity before the test, as this can affect the hemoglobin levels in your blood and the test results.
  • Inform your healthcare provider if you have a bleeding disorder or are taking blood-thinning medication, as this may affect the blood draw and the test results.

What Happens During Hemoglobin Variant Analysis Testing?

A HPLC Blood Test only takes a few minutes.

  • As per the scheduled time of the blood test, a lab technician who usually takes blood samples will arrive.
  • A thin needle will be used to draw blood from your arm's vein.
  • The needle might cause a mild pinch and some discomfort.
  • The lab technician then fills a collection tube with blood and then removes the needle from the skin.
  • They place a small bandage on the arm.

Should you book blood test online to check your HPLC levels or go to the nearest clinic and get it done by a medical professional? Let's Find Out.

Can I Take HPLC Blood Test At Home?

No, you cannot take the HPLC blood test at home because the test requires specialized equipment and trained medical professionals to draw and process the blood sample. The test involves separating and identifying different types of hemoglobin in your blood based on their size and electrical charge, which requires using a high-performance liquid chromatography (HPLC) machine. 

Collecting the blood sample in a sterile environment is vital to reduce the risk of infection. Therefore, you will need to visit a healthcare provider or a laboratory to have the HPLC blood test performed.

How Much Does HPLC Test Cost?

HPLC blood test cost varies depending on several factors, like your location, the healthcare provider or the laboratory from which you will get the test done. Your health insurance coverage also plays a major role in this, and you need to check with them.

You received your HPLC test report but still need help determining if you have antibodies in the normal range. Read this section to understand how to go about your antibody diagnoses with your healthcare professional.

What Do HPLC Blood Test Results Mean?

The results of the HPLC blood test will provide information about the different types of hemoglobin in your blood. The results may indicate the following:

  • The presence of abnormal hemoglobin variants: The HPLC machine will separate and identify different types of hemoglobin based on their size and electrical charge. If any abnormal hemoglobin variants are present in your blood, the HPLC machine will be able to detect them.
  • The type and severity of any hemoglobin disorders: Based on the test results, your healthcare provider will be able to diagnose and classify any hemoglobin disorders you may have, such as sickle cell anemia or thalassemia. They may also be able to determine the severity of your condition and develop an appropriate treatment plan.
  • Your risk of passing on a hemoglobin disorder to your children: If you have a hemoglobin disorder, the HPLC blood test can help determine your risk of passing on the disorder to your children. It can be helpful information if you are planning to start a family.

What Is HPLC Blood Test Normal Range?

Hemoglobin (Hb) Normal Range

Pregnant Females

11 g/dL - 16 g/dL

Non-Pregnant Females

12.0 g/dL - 15.5 g/dL

What Medical Conditions Can Cause High or Low HPLC Levels?

Certain medical conditions may cause abnormal hemoglobin variants in your blood, which the HPLC blood test can detect. These conditions may include the following:

  • Sickle cell anemia
  • Thalassemia
  • Hemoglobin C disease
  • Hemoglobin D disease
  • Hemoglobin E disease
  • Hemoglobin H disease
  • Hemoglobin Lepore syndrome
  • Hemoglobin S-C disease

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Disclaimer: Although we have endeavored to provide accurate information on this page, we strongly recommend that you seek advice from your doctor before relying on any of the test ranges or lab-test recommendations mentioned herein.

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FAQs Around HPLC Blood Test

The HPLC blood test is conducted during pregnancy to screen for certain hemoglobinopathies (inherited blood disorders such as sickle cell anemia or thalassemia) that can affect the health of both the mother and the baby.

An HPLC blood test can identify and measure the different types of hemoglobin in your blood. This test can also diagnose certain medical conditions or determine your risk of passing on a hemoglobin disorder to your children.

If your HPLC levels are high, you may have a hemoglobin disorder or another medical condition affecting your blood. Your healthcare provider will interpret your HPLC results in the context of your medical history and symptoms to determine the underlying cause of the high levels.

HPLC is a blood test to identify & measure hemoglobin types in your blood. So, no specific symptoms directly associate with HPLC. Still, if the HPLC test detects an abnormal hemoglobin variant or disorder, the symptoms may include fatigue, shortness of breath, pale skin, dizziness, etc.

Beta thalassemia is a genetic blood disorder that affects the production of beta-globin chains in the hemoglobin protein. You cannot cure it, but proper treatment can help you manage the symptoms and improve your quality of life.

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  • Disclaimer: HealthcareOnTime offers extensively researched information, including laboratory testing for health screening. However, we must emphasize that this content is not intended as a substitute for professional medical advice or diagnosis. Always prioritize consulting your healthcare provider for accurate medical guidance and personalized treatment. Remember, your health is of paramount importance, and only a qualified medical professional can make precise determinations regarding your well-being. Protection Status Protection Status Protection Status