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Vitamin D Test: Costs, Purpose, Normal Range and Interpretation

Vitamin D Test
  • Sample Type : Blood
  • Parameters : 1
  • Report Time : 24/48 Hrs
  • Home Collection: Yes
  • Fasting : Not Required
  • Age Group: All
OFFER PRICE:  ₹970 ₹1300 30% off


Vitamin D Test
58+ booked in last 3 days

A Vitamin D Deficiency Test, also known as a 25-OH Cholecalciferol, 25-OH Vitamin D Test, Calcidiol Test, or Vitamin D3 Test, measures your body's Vitamin D levels to assess overall bone health and immune system function.

It measures the concentration of 25-hydroxyvitamin D, the major circulating form of vitamin D in the body, studying which allows valuable insights into vitamin D levels and helps healthcare professionals determine if there is a vitamin D deficiency or not. The two most common forms of vitamin D that are tested are 25-hydroxyvitamin D2 (25(OH)D2) and 25-hydroxyvitamin D3 (25(OH)D3). These are the major circulating forms of vitamin D in the body and serve as indicators of an individual's vitamin D status.

Types of Vitamin D Test

  • 25-Hydroxy Vitamin D Test (25-OH Vitamin D Test): This is the most common and comprehensive test, measuring both Vitamin D2 and D3 levels.
  • Vitamin D2 Test: Specifically measures Vitamin D2 levels, which come from plant-based sources and supplements.
  • Vitamin D3 Test: Specifically measures Vitamin D3 levels, the more biologically active form synthesized by the skin and found in some animal-based foods.
  • 1,25-Dihydroxy Vitamin D Test (1,25-OH Vitamin D Test): Measures the active form of Vitamin D, typically used for specific medical conditions.

These tests help assess Vitamin D status and guide appropriate interventions to maintain health. In practice, when you request a "Vitamin D Deficiency Test" from your healthcare provider, they will usually measure both Vitamin D2 and Vitamin D3, giving you a total Vitamin D level. This is because both forms are important for assessing your overall Vitamin D status. However, if you have a specific need to measure just one form, you can request to focus on that particular form.

Learn everything about Vitamin D testing before you book lab test online. Understand the why, how, and what of this test. 

Why Do I Need Vitamin D Deficiency Testing?

The body needs optimal levels of vitamin D is essential for maintaining the overall health and well-being of an individual. Hence, the Vitamin D Level  test helps provide valuable insights into the current vitamin D levels and helps determine if they fall within the vitamin D normal range. By assessing the concentration of 25-hydroxyvitamin D, healthcare professionals can identify deficiencies and address them accordingly.

Here are some major reasons why you may need vitamin D testing:

  • Assessing vitamin D levels
  • Identifying deficiency symptoms
  • Evaluating bone health
  • Managing chronic diseases
  • Assessing dietary deficiencies
  • Monitoring supplementation
  • Determining appropriate supplementation dosage

How To Prepare For Vitamin D Blood Test?

There are no special preparations needed when getting a Vitamin D blood test. In certain cases, the healthcare provider might want you to fast (not consume any food for a few hours) before the test.

What Happens During Vitamin D Testing

A Vitamin D Blood test only takes a few minutes. 

  • As per the scheduled time of the blood test, a lab technician who usually takes blood samples will arrive.
  • A thin needle will be used to draw blood from your arm’s vein.
  • The needle might cause a mild pinch and some discomfort.
  • The lab technician then fills a collection tube with blood and then removes the needle from the skin.
  • They place a small bandage on the arm.

Should you book blood test online to check your Vitamin D levels or go to the nearest clinic and get it done by a medical professional? Let's Find Out.

Can I Take Vitamin D Test At Home?

Yes, the Vitamin D blood test can be taken at home. HealthcareOnTime’s at-home lab testing service, in association with Thyrocare, provides a convenient and efficient way to get important medical tests done from the comfort of your place without the need for a doctor's visit or a trip to a lab. It is always preferable to consult with a healthcare provider about any concerns regarding your test results.

How Much Does Vitamin D Test Cost?

Are you curious about the Vitamin D test price? At HealthcareOnTime, we offer a budget-friendly solution to ensure you get the vital information you need about your health.

Vitamin D blood test cost can vary depending on your location, the healthcare provider, and your insurance coverage. However, our website provides clarity – you can access this crucial test for just INR 970! What's more, our prices are not only competitive but also maintain the highest quality standards.

In India, Vit D test cost generally start from '649, but these figures can fluctuate depending on the laboratory and city. Given that around 8 out of 10 people in India are vitamin D deficient, it's a test often recommended by doctors to assess your vitamin D levels.

But here's the exciting part – HealthcareOnTime goes above and beyond to make healthcare accessible. We offer an incredible up to 30% discount on Vitamin D tests in India. This service is powered by our trusted partner, Thyrocare, and is available in over 4500+ pincodes nationwide. Plus, we make it hassle-free with free home sample collection.

You can also explore our full range of all vitamin test packages and get your most common vitamin deficiency checked by healthcareOnTime.

Test Name

Cost (₹)

Vitamin Profile Test - Basic (2 parameters) 


Complete Vitamin Profile Test - Advanced (14 parameters) 


Vitamin B-12 Test


Vitamin A Deficiency Test


25-Oh Vitamin D Test


Folate Test


Vitamin K Test


Vitamin E Test


Vitamin D 1,25 - Dihydroxy Test


You received your Vitamin D blood test results but still need help determining if they fall under the normal range. Read this section to know if your results range between the Vitamin D normal range.

What Do Vitamin D Test Results Mean?

A vitamin D Level test gives one of the following 3 results for your healthcare provider to study and create a diagnosis upon:

  • Within Normal Range: This indicates a healthy vitamin D status.
  • Below Normal Range: Such a result may indicate a potential vitamin D deficiency.
  • Above Normal Range: Such a rare instance can be attributed to various factors, such as certain medical conditions or excessive supplementation.

Remember, accurate interpretation of vitamin D checkup results is pivotal for effective management and optimal health. Consult with your healthcare provider for personalized advice and appropriate interventions.

What Is Vitamin D Test Normal Range?

Refer to the table below for Vitamin D test ranges. 

Normal Range 

50 nmol/L (20 ng/mL) - 125 nmol/L (50 ng/mL)


30 nmol/L (12 ng/mL) -  50 nmol/L (20 ng/mL)

What Medical Conditions Can Cause High Vitamin D Levels?

While vitamin D deficiency is commonly discussed, it is important to acknowledge that certain medical conditions can lead to abnormally high vitamin D levels as well.

  • Excessive Vitamin D Supplementation
  • Overconsumption of Fortified Foods
  • Granulomatous disorders
  • Genetic Disorders
  • Sarcoidosis
  • Lymphoma
  • Hypercalcemia
  • Inflammatory Bowel Disease
  • Kidney Disease

What Medical Conditions Can Cause Low Vitamin D Levels?

There are various factors that can lead to Vitamin D deficiency in one’s body, such as:

  • Inadequate Sun Exposure
  • Osteoporosis
  • Renal Insufficiency (kidney diseases)
  • Malabsorption Syndromes (e.g., celiac disease, Crohn's disease)
  • Liver Disease  (e.g., cirrhosis, hepatitis)
  • Hyperparathyroidism
  • Obesity

How Is Vitamin D Deficiency Treated And Managed?

  • Management of Vitamin D Deficiency: the management of Vitamin D deficiency involves addressing the underlying causes and making lifestyle modifications. This includes increasing sun exposure in a safe manner, as sunlight is a natural source of Vitamin D. Additionally, incorporating Vitamin D-rich foods into the diet, such as fatty fish and fortified dairy products, can help maintain adequate levels. Regular monitoring of Vitamin D levels and adherence to treatment and lifestyle recommendations are key to effectively managing Vitamin D deficiency.
  • Treatment of Vitamin D Deficiency: For mild deficiencies, oral Vitamin D supplements may be prescribed. In more severe cases, higher doses or injections of Vitamin D may be recommended to rapidly increase levels. It is essential to follow the prescribed treatment plan and regularly monitor Vitamin D levels to ensure appropriate supplementation.

There are various risk factors a person develop due to Vitamin D deficiency, such as:

  • Weakened bone health
  • Osteoporosis
  • Muscle weakness
  • Increased risk of falls
  • Impaired immune function
  • Increased risk of chronic diseases
  • Impaired mood and mental health

Convenience at Your Doorstep

Ever wished for healthcare that comes to you? HealthcareOnTime makes it a reality with doorstep lab testing, cutting out clinic hassles. No more queues or travel stress. Experience at-home sample collection, prioritizing health without time constraints. —your path to health, now just a doorstep away!

Affordable Testing with Thyrocare Partnership

Experience cost-effective lab testing at-home with HealthcareOnTime's exclusive partnership with Thyrocare. Benefit from competitive prices while ensuring precise results. Our collaboration with Thyrocare Technologies Limited guarantees affordability without compromising on the accuracy and reliability of your lab test.

Comprehensive Health Screening

At HealthcareOnTime, we've got your back with our comprehensive health checkup packages! Take charge of your well-being by booking online. These packages empower you to stay ahead, catching potential issues early for timely management. It's like having a health ally, and making informed decisions for a healthier, happier life.

Disclaimer: Although we have endeavored to provide accurate information on this page, we strongly recommend that you seek advice from your doctor before relying on any of the test ranges or lab-test recommendations mentioned herein.


Ref Links: 

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Test of value less than Rs.299 will cost Rs 200 extra towards collection charges

FAQs Around Vitamin D Blood Test

Vitamin D tests can be conducted at any time of the day with no restrictions.

Age in Years



15 mcg (600 IU)


15 mcg (600 IU)


15 mcg (600 IU)


20 mcg (800 IU)

Diseases like osteoporosis, kidney disease, liver disease, inflammatory bowel disease, and certain autoimmune disorders can lead to Low Vitamin D Levels.

No, a vitamin D test does not require fasting or an empty stomach. It can be done at any time of the day.

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  • Disclaimer: HealthcareOnTime offers extensively researched information, including laboratory testing for health screening. However, we must emphasize that this content is not intended as a substitute for professional medical advice or diagnosis. Always prioritize consulting your healthcare provider for accurate medical guidance and personalized treatment. Remember, your health is of paramount importance, and only a qualified medical professional can make precise determinations regarding your well-being. Protection Status Protection Status Protection Status